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Substrate Services Core Research Support

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Overview

The Substrate Services Core Research Support (SSCRS) was developed in 2014 to serve as a centralized processing core and storehouse for research samples for large clinical, consortium-based collaborations and for basic, discovery science. We meet the increasing need for high-quality, well-characterized biological samples to be utilized by researchers working in clinical and translational research, ultimately enhancing the diagnosis and treatment of future patients.

The SSCRSS produces quality translational science through validated, standardized methodology and protocols that are implemented across studies, reducing non-relevant variability in results. To support this standardization, the core is in compliance with internationally recognized Good Clinical Laboratory Practice (GCLP) and 21 CFR Part 11. Reproducibility across supported research is achieved through continued monitoring of the quality system by the Quality Assurance for Duke Vaccine Immunogenicity Programs (QADVIP), an independent quality assurance unit.

Graphic displaying the discovery, development, and delivery process

Available Services

 

All services provided adhere to Good Clinical Laboratory Practice (GCLP).

  • Collection and processing of tissue biopsies, serum, plasma, PBMCs, whole blood, urine, and various other biospecimens
  • On-call technician for sample processing for collections that occur outside of business hours
  • Isolation of DNA or RNA from cells, whole blood, or tissue
  • Patient/sample de-identification utilizing 2D-coded cryovial technology
  • Sample inventory system for reporting and rapid retrieval of samples
  • Secure monitored cryogenic long-term storage
  • Transfer, shipping, and tracking of samples
  • Data management
  • Clinical data linking
  • mRNA & miRNA expression
  • Histology services
    • Complete routine paraffin processing, embedding, and sectioning
    • Fresh tissue freezing, embedding, and sectioning
    • Basic histological stains, special stains, and immunohistochemical stains on paraffin and frozen sections

Equipment and Facilities

We are located on the 4th floor of the Medical Research Building I (MSRB1), which includes approximately 1,150 square feet of dedicated laboratory space for sample processing and analysis, and an additional 800 square feet of secured, dedicated freezer space. The laboratory is outfitted with:

  • 10 upright -80C freezers
  • 2 cryogenic -150C freezers
  • 2 liquid nitrogen cryogenic freezers
  • 1 upright -20C freezer
  • 3 4c refrigerators for reagent storage
  • 2 Countess II automated cell counters
  • 2 Leica cryostats
  • 1 TBS ATP1 Automated Tissue Processor
  • 1 TBS Tissue Embedding Station
  • 3 paraffin sectioning microtomes
  • 2 tissue homogenizers
  • 3 tissue floatation baths
  • 3 histology staining stations
  • 3 stage microscopes
  • 1 fluorescent microscope
  • 1 Leica Aperio Digital Pathology Slide Scanner
  • 1 HTG EdgeSeq System
  • 1 Qiagen QIAcube Workstation for automated purification of DNA, RNA, or proteins
  • 1 BD Phoenix™ Automated Microbiology System
  • 1 Bio-Rad Bio-Plex 200 suspension array system

Lab member holding a histology staining slide

Members

 

Jennifer Cheeseman-Janes, MS, Senior Director

Jennifer has over 14 years of experience in the scientific and technical management of clinical, basic science, and translational research, and has extensive knowledge in proposal development, financial planning, project management, regulatory and institutional compliance, and improvement of organizational efficiency within the academic setting at both divisional and departmental levels. She has spent the last 3 years at Duke University developing research support via core services, improving and developing core infrastructure, improving long-term strategic planning and promotion of collaborative and transformative research with collaborators both internally and externally. She serves as the biobanking lead of two Department of Defense biobanking consortiums in collaboration with Duke University, the Department of Surgery, and the Surgical Critical Care Initiative (SC2i), supporting precision medicine in wounded warriors and the Vascular Composite Allograft Collaborative Initiative (VCAci), supporting translational research in hand transplantation. Prior to joining Duke, Jennifer served as the Director of the Emory University Transplant Center Biorepository where she developed and implemented an Integrated Research Support Service that encompassed study development around sample and clinical data collection and linking, financial planning, project management, clinical regulatory and institutional compliance, improving on organizational efficiency within the academic setting at both divisional and departmental levels. Jennifer earned her BS and MS from the University of Florida and is currently completing her MBA at North Carolina State University.

Mary-Beth Joshi, MPH, Assistant Director

Mary-Beth has spent more than 20 years directing complex, multi-faceted, multi-institutional, and multi-disciplinary clinical, translational, and basic science as well as clinical outcomes research projects in oncology. For more than two decades she has been instrumental in the development, implementation, and management of biospecimen and clinical data repositories and associated experimental data with the ability to transform working hypotheses into fully executable projects with defined benchmarks, goals, and measurable objectives. She has extensive experience in operations management effectively streamlining and optimizing project processes and resources to meet defined benchmarks and timelines. Mary-Beth earned her BS in Biology and Sports Medicine from Wingate College and her MPH in Public Policy from AT Still University.

  • Robyn Osborne, MS, Laboratory Manager
  • Debbi Conlon, HT, Laboratory Manager
  • Julie Fuller, Histology Lead
  • Bartley Adams, Molecular Lead

Group photo of SSCRS faculty and staff

Selected Achievements