Duke Immune Profiling Core (DIPC)

DIPC logo

Overview

The Duke Immune Profiling Core (DIPC) is a Duke School of Medicine and Duke Cancer Center Institute Service Center whose academic home resides in the Department of Surgery. Our overarching mission, "To identify immunologic signatures that predict clinical outcomes," comprises two components. The first is a Service component in which DIPC provides comprehensive, highly standardized, state-of-the-art human immunologic profiling services in support of both intra- and extramural translational research endeavors, including Phase I/II clinical trials. The second DIPC component focuses on Discovery, and utilizes advanced, high-dimensional Core technologies and resources to stimulate novel translational research initiatives that will impact the existing "standard of medical care." With over 28 years of experience in flow cytometry and immune profiling, we are available to support a wide range of services, such as small-scale projects for generating pilot data extending through clinical trial testing. Additionally, our services extend to single-cell proteomics and multiplex immunoassays, study planning, performing experiments, data collection and analysis, and writing support.

Available Services

FY2023 Rates

 

Service Provided

Internal Duke Users

External,

Higher-Education

Users*

All Other External Users*

Unit

 

Reagents & Consumables

 

##Flow Cytometry Staining

$16.50

$26.57

$53.14

Per Tube

 

 

 

Luminex/ELISA Assay

$99.00

$159.39

$318.78

Per Tube

Equipment

$$BD LSRFortessa/FACS Symphony A5 (X50)

$150.00

$241.50

$483.00

$%Per Hour

 

Luminex

$150.00

$241.50

$483.00

Per Plate

 

IsoLight

$150.00

$241.50

$483.00

Per Run

Technical Services

^^Technician/Core Operator

$115.00

$185.15

$370.30

Per Hour

Bio-statistical Support

Bio-statistics and Computational Biology

$94.00

$151.34

$302.68

Per Hour

Thawing

Reagents & Supplies for Cell Thawing (& Overnight Resting)

$19.00

$30.59

$61.18

Per Vial

Counting

Reagents & Supplies for Cell Counting (Automated)

$2.50

$4.03

$8.06

Per Sample

Stimulation

&&Reagents & Supplies for Cellular Stimulation

$4.50

$7.25

$14.50

Per Sample stimulation condition

Sample Isolation (CD45, CD4, CD8, etc)

!!Reagents & Supplies for Sample Isolation

$27.00

$43.47

$86.94

Per Sample isolation condition

Sample Processing

**PBMC, Lymph Node, Ascites, and Plueral Effusion Processing

$44.00

$70.84

$141.68

Per Sample

Tumor Processing

%%Tumor and Biopsy Processing

$135.00

$217.35

$434.70

Per Sample

Other Services

Sample Pick-up/Delivery

$115.00

$185.15

$370.30

Per Hour

 

Leukapheresis Batch Control cryovials

$5.00

$8.05

$16.10

Per Vial

 

Specific mAb, buffers, peptides, etc necessary for performing assays

Market Price

Market Price

Market Price

Market Price

 

*External prices include a mandatory overhead charge.  Overhead for internal users is not shown but is taken from the indirect costs of their funding source.

##Per Tube charge is a minimum base rate for single color.  Rate increases based on whether Investigator or DIPC provides consumables (ie tubes, tips, plates, etc), Red Cell lysing, Cell Permeabilization Buffers, and/or quantitation.

$$BD LSR Fortessa and FACS Symphony A5 (X50) usage is charged by the hour with exception to the user that has a TOTAL acquisition time of 30 minutes or less. 

$%A complete acquisition of 30 minutes or less will be charged $75.00, as approved by the SOM.

^^Technician/Core Operator time is charged by the 0.5hr.  Any time between 1-30 minutes = 0.5hr, 31-60 minutes = 1hr.

&&Cellular Stimulation is for 1 stimulation.  The costs include consumables and reagents except for the test antigen(s).  Technician time will be applied as listed above.

!!Sample Isolation is for 1 sample isolation for CD45, CD4, CD8, etc cells.  The costs include consumables and reagents necessary for sample isolation.  Technician time will be applied as listed above.

**PBMC, Lymph Node, Ascites, and Plueral Effusion Processing costs include consumables and reagents.

%%Tumor and Biopsy Processing costs include consumables and reagents including Tumor Dissociation Kit Enzymes. Technician time will be applied as listed above.

Equipment and Facilities

DIPC Photo
BD SORP Symphony A5 (44 colors)

 

DIPC photo
BD SORP LSRFortessa (18 colors)
DIPC photo
Luminex MAGPIX

DIPC Leadership

DIPC Staff

  • Jennifer Enzor
  • Twan Weaver
  • Preksha Patel
  • Katelyn Steadman
  • Erin Murray

DIPC team

Selected Achievements

Immune profiling for vaccine trials

  • From a multimillion dollar grant from NIH, DIPC will assess the cellular immune responses of volunteers in a malaria vaccine trials through the Vaccine Trials Evaluation Unit (VTEU).
     

Immune profiling for cancer immunotherapy trials

  1. Neoadjuvant ipilimumab therapy for non-small cell lung cancer (NSCLC); NCT01820754
  2. Neoadjuvant Pembrolizumab therapy or non-small cell lung cancer; NCT02818920
  3. Evaluation of nivolumab alone versus nivolumab + ipilimumab therapy for recurrent glioblastomas; NCT02017717
  4. Treatment of urothelial cancers with pembrolizumab alone versus pembrolizumab + ACP196 (a BTK inhibitor); IND#124755
  5. Evaluation of anti-tumor reactivities in the lymph node and PBMC of metastatic, castrate-resistant prostate cancer patientsbefore or after receiving treatment with sipuleucel-T; NCT02036918NCT02036918
  6. A randomized trial of Pembrolizumab and radiotherapy verus radiotherapy in high-risk soft tissue sarcoma of the extremity; NCT03092323

Collaborate with US

Core Staff Contact

duke.ipc@dm.duke.edu
Jennifer Enzor: (919) 684-3315
Katelyn Steadman: (919) 684-3542

Surgical Oncology Research Facility (SORF)
915 South LaSalle Street Ext.
Room 120 / 122 / 124
Durham, NC 27710

DIPC Map