Skip to main content

Kent James Weinhold, PhD

Joseph W. and Dorothy W. Beard Distinguished Professor of Experimental Surgery, in the School of Medicine
Chief, Division of Surgical Sciences
Director, Laboratories/Surgical Sciences, Basic Research
Professor of Surgery
Professor of Immunology
Professor in Pathology
Office: 204 Surg Oncol Res Fac, Durham, NC 27710
Campus Mail: DUMC Box 2926 Med Ctr, Durham, NC 27710

In addition to their ongoing HIV/AIDS-related research activities, the Weinhold Laboratory is focused on utilizing a comprehensive repertoire of highly standardized and formerly validated assay platforms to profile the human immune system in order to identify immunologic signatures that predict disease outcomes. These ongoing studies span a broad range of highly relevant clinical arenas, including: 1) cancer (non-small cell lung cancer, head and neck cancer, glioblastoma neoforme, ovarian cancer, and prostate cancer), 2) autoimmune diseases (rheumatoid arthritis, systemic lupus erythematosis, multiple sclerosis, and myasthenia gravis), 3) pulmonary disease (idiopathic pulmonary fibrosis), 4) solid organ transplantation (lung, kidney, liver, and heart), and 5) rare diseases (Pompe disease).

Two of the areas that have been especially active over the past few years include the comprehensive immunologic profiling of cancer patients receiving so-called ‘immune checkpoint blockade’ therapies and the search for immune signatures in lung transplant recipients that track with resistance to CMV infection. The laboratory is conducting immune monitoring studies associated with a Phase I trial of Ipilimumab (anti-CTLA-4) in a neoadjuvant setting for the treatment of non-small cell lung cancer (NSCLC). For this trial we are extensively utilized several polychromatic flow cytometry (PFC) platforms to follow activation, maturation, exhaustion, and proliferation patterns within CD4+ and CD8+ subsets of T-cells. We are also utilizing an intracellular cytokine staining (ICS) platform in efforts to detect anti-tumor associated antigen (TAA) responses by CD4+ and CD8+ T cells from peripheral blood mononuclear cells as well as lymphocytes infiltrating the patients’ tumor. These assays are designed to measure antigen-driven intracellular production of IFN-γ, TNF-α, and IL-2, as well as the degranulation marker CD107. This strategy enables us to not only document individual cytokine responses, but to also assess (through Boolean gating) changes in relative polyfunctionality of the responses. We are performing similar immune monitoring of a Phase II trial evaluating nivolumab (anti-PD-1) alone vs. combined nivolumamb + ipilimumab vs. avastin (bevacizamab) alone in patients with glioblastomas. In both studies, we are seeking to identify pharmacodynamics markers and immune correlates predictive of clinical responses. In recently completed studies in a cohort of lung transplant recipients, we identified specific polyfunctional signatures in CD4+ and CD8+ subsets against CMV pp65 and IE-1 antigens that tracked with resistance to CMV infection (manuscript in preparation). These findings now serve as the basis for a Phase I clinical trial to compare conventional 6-month chemoprophylaxis in lung transplant recipients versus a regimen dictated by the presence or absence of the predictive signatures. This trial is the principal component of a recently awarded Clinical Trials in Organ Transplantation or CTOT award made from the NIH to Duke (Scott Palmer, PI). Ongoing studies will test the hypothesis that these signatures that have been validated in lung transplant recipients will also predict resistance to CMV infection in the context of other solid organ transplants such as kidney, liver, and heart.

Future studies will also attempt to identify predictive signatures for resistance to BK polyomavirus, the cause of graft threatening nephritis in kidney transplant recipients and cystitis in bone marrow transplant recipients. Other human diseases that are presently being subjected to comprehensive immune profiling by the laboratory include idiopathic pulmonary fibrosis (IPF), myasthenia gravis, multiple sclerosis, systemic lupus erythematosus, acute coronary syndrome, and Pompe disease

 

 

Recent publications

Zidar, D.A., Mudd, J.C., Juchnowski, S., Lopes, J.P., Sparks, S., Park, S.S., Ishikawa, M., Osborne, R., Washam, J.B., Chan, C., Funderburg, N.T., Owoyele, A., Alaiti, M.A., Mayuga, M., Orringer, C., Costa, M.A., Simon, D.I., Tatsuoka, C., Califf, R.M., Newby, L.K., Lederman, M.M., and Weinhold, K.J.  Altered maturation status and possible immune exhaustion of CD8 T lymphocytes in the peripheral blood of patients presenting with acute coronary syndromes. Arterioscler., Thromb., and Vasc. Biol. 36(2): 389-397, Feb. 2016 PMID: 26663396

Yi, J.S., Russo, M.A., Weinhold, K.J., and Guptill, J.T. Adaptive immune response to therapy in HMGCR autoantibody myopathy. Muscle Nerve.  53(2): 313-317, Feb. 2016. PMID: 26492512          

Snyder, L.D., Chan, C., Kwon, D., Yi J.S., Martissa, J.A., Copeland, C.A.F., Osborne, R.J., Sparks, S.D., Palmer, S.M., Weinhold, K.J.  Polyfunctional T cell responses predict protection from cytomegalovirus after lung transplant.  Am J Respir Crit Care Med. 193 (1): 78-85, Jan.1, 2016 [PMID 26372850]

Guptill, J.T., Yi, J.S., Sanders, D.B., Guidon, A.C., Juel, V.C., Massey, J.M., Howard, J.F., Jr., Scuderi, F., Bartoccioni, E., Evoli, A. and Weinhold, K.J. “Characterization of B cells in muscle-specific kinase antibody myasthenia gravis. Neurol Neuroimmunol Neuroinflamm. 2015 Feb 26;2(2) e77. [PMID 25745635]

Staats, J.S., Enzor, J.H., Sanchez, A.M., Roundtree, W., Guar, A., Jaimes, M., Denny, T.N., and Weinhold, K.J. “Toward a comprehensive external quality assurance program for polychromatic flow cytometry.” J. Immunol. Methods. 409:44-53, 2014. [PMID 24968072]

Nair, S.K., Tomaras, G.D., Sales A.P., Boczkowski, D., Chan, C., Plonk, K., Dannull, J., Pruitt, S.K., and Weinhold, K.J. “High-throughput identification and dendritic cell-based functional validation of MHC class I-restricted Mycobacterium tuberculosis epitopes.” Scientific Reports. Apr. 23, 2014. [PMID 24755960]

Yi, J.S., Guidon, A., Sparks, S., Osborne, R., Juel, V.C., Massey, J.M., Sanders, D.B., Weinhold, K.J., and Guptill, J.T. “Characterization of CD4 and CD8 T cell responses in MuSK myasthenia gravis.” Journal of Autoimmunity. 2013 Dec27 doi:1016/j.jaunt2013. 12.005. [PMID: 24378287]

Education and Training

  • Ph.D., Thomas Jefferson University, 1979

In the News

Selected Grants

Publications

Zhang, Tian, Michael R. Harrison, Peter H. O’Donnell, Ajjai S. Alva, Noah M. Hahn, Leonard J. Appleman, Jeremy Cetnar, et al. “A randomized phase 2 trial of pembrolizumab versus pembrolizumab and acalabrutinib in patients with platinum-resistant metastatic urothelial cancer.” Cancer, August 5, 2020. https://doi.org/10.1002/cncr.33067.

Full Text

Nobuhara, Chloe K., W Michael Bullock, Thomas Bunning, Brian Colin, Mary Cooter, Michael J. Devinney, Michael N. Ferrandino, et al. “A protocol to reduce self-reported pain scores and adverse events following lumbar punctures in older adults.” J Neurol 267, no. 7 (July 2020): 2002–6. https://doi.org/10.1007/s00415-020-09797-1.

Full Text

Healy, Zachary R., Kent J. Weinhold, and David M. Murdoch. “Transcriptional Profiling of CD8+ CMV-Specific T Cell Functional Subsets Obtained Using a Modified Method for Isolating High-Quality RNA From Fixed and Permeabilized Cells.” Front Immunol 11 (2020): 1859. https://doi.org/10.3389/fimmu.2020.01859.

Full Text

Berger, Miles, David M. Murdoch, Janet S. Staats, Cliburn Chan, Jake P. Thomas, Grant E. Garrigues, Jeffrey N. Browndyke, et al. “Flow Cytometry Characterization of Cerebrospinal Fluid Monocytes in Patients With Postoperative Cognitive Dysfunction: A Pilot Study.” Anesth Analg 129, no. 5 (November 2019): e150–54. https://doi.org/10.1213/ANE.0000000000004179.

Full Text

Nyanhete, Tinashe E., Alyse L. Frisbee, Todd Bradley, William J. Faison, Elizabeth Robins, Tamika Payne, Stephanie A. Freel, et al. “HLA class II-Restricted CD8+ T cells in HIV-1 Virus Controllers.” Sci Rep 9, no. 1 (July 15, 2019): 10165. https://doi.org/10.1038/s41598-019-46462-8.

Full Text

Schroder, P., J. S. Yi, K. J. Weinhold, C. Chan, M. Joshi, C. Walters, J. Kwun, and S. J. Knechtle. “Pre-Transplant Multidimensional Flow Cytometric Analysis of Kidney Transplant Recipients Reveals Novel Immune Signature of Allograft Rejection.” In American Journal of Transplantation, 19:765–66. WILEY, 2019.

Scholars@Duke

Berger, Miles, Deborah Oyeyemi, Mobolaji O. Olurinde, Heather E. Whitson, Kent J. Weinhold, Marty G. Woldorff, Lewis A. Lipsitz, et al. “The INTUIT Study: Investigating Neuroinflammation Underlying Postoperative Cognitive Dysfunction.” J Am Geriatr Soc 67, no. 4 (April 2019): 794–98. https://doi.org/10.1111/jgs.15770.

Full Text

Zhang, Tian, Janet S. Staats, Cliburn Chan, Ajjai Shivaram Alva, Peter H. O’Donnell, Michael Roger Harrison, Kristen A. Batich, Veerendra Munugalavadla, Daniel J. George, and Kent J. Weinhold. “Multidimensional flow characterization of circulating immune cells in cisplatin-resistant metastatic urothelial cancer (mUC) patients (pts) treated with pembrolizumab (P) with or without acalabrutinib (acala).” In Journal of Clinical Oncology, 37:357–357. American Society of Clinical Oncology (ASCO), 2019. https://doi.org/10.1200/jco.2019.37.7_suppl.357.

Full Text

Yi, John S., Marilyn Rosa-Bray, Janet Staats, Pearl Zakroysky, Cliburn Chan, Melissa A. Russo, Chelsae Dumbauld, et al. “Establishment of normative ranges of the healthy human immune system with comprehensive polychromatic flow cytometry profiling.” Plos One 14, no. 12 (2019): e0225512. https://doi.org/10.1371/journal.pone.0225512.

Full Text

Woroniecka, Karolina, Pakawat Chongsathidkiet, Kristen Rhodin, Hanna Kemeny, Cosette Dechant, S Harrison Farber, Aladine A. Elsamadicy, et al. “T-Cell Exhaustion Signatures Vary with Tumor Type and Are Severe in Glioblastoma.” Clin Cancer Res 24, no. 17 (September 1, 2018): 4175–86. https://doi.org/10.1158/1078-0432.CCR-17-1846.

Full Text

Pages