Skip to main content

David Charles Montefiori, PhD

Professor of Surgery
Office: Surgical Oncology Res Fac, Durham, NC 27710
Campus Mail: DUMC Box 2926 Med Ctr, Durham, NC 27710

Dr. Montefiori is Professor and Director of the Laboratory for AIDS Vaccine Research and Development in the Department of Surgery, Division of Surgical Sciences, Duke University Medical Center. His major research interests are viral immunology and AIDS vaccine development, with a special emphasis on neutralizing antibodies. One of his highest priorities is to identify immunogens that generate broadly cross-reactive neutralizing antibodies for inclusion in HIV vaccines.  Many aspects of the neutralizing antibody response are studied in his laboratory, including mechanisms of neutralization and escape, epitope diversity among genetic subtypes and geographic origins, ontogeny of broadly neutralizing antibody responses and computationally-assisted vaccine design. Over the years he has explored multiple types of assays for neutralizing antibodies and other potential antiviral antibodies, focusing on assay optimization, standardization, validation and high throughput. The scope of research covers HIV-infected individuals and nonhuman primate models of simian immunodeficiency virus (SIV) and simian-human immunodeficiency virus (SHIV) infection.  Dr. Montefiori also directs a large AIDS vaccine immune monitoring program, which operates in compliance with Good Clinical Laboratory Practice and has served as a national and international resource for standardized assessments of neutralizing antibody responses in preclinical and clinical trials of candidate AIDS vaccines since 1988. In 2002 he chaired the Antibody Laboratory Standardization Working Group that assisted in the design of the Global HIV Vaccine Enterprise Strategic Plan for HIV vaccines. He currently directs the Core Neutralizing Antibody Laboratories for the HIV Vaccine Clinical Trials Network (HVTN), the NIH Preclinical HIV-1 Vaccine Program and the Center for HIV/AIDS Vaccine Immunology- Immunogen Design (CHAVI-ID). He also directs a large Comprehensive Antibody Vaccine Immune Monitoring Consortium (CAVIMC) as part of the Bill and Melinda Gates Foundation's Collaboration for AIDS Vaccine Discovery (CAVD). He has published over 500 original research papers that have helped shape the scientific rationale for antibody-based HIV-1 vaccines.

Education and Training

  • Ph.D., Clemson University, 1982

Publications

Rathore, Ujjwal, Mansi Purwar, Venkada Subramanian Vignesh, Raksha Das, Aditya Arun Kumar, Sanchari Bhattacharyya, Heather Arendt, et al. “Bacterially expressed HIV-1 gp120 outer-domain fragment immunogens with improved stability and affinity for CD4-binding site neutralizing antibodies.” J Biol Chem 293, no. 39 (September 28, 2018): 15002–20. https://doi.org/10.1074/jbc.RA118.005006.

Full Text

Singh, Shakti, Eric G. Ramírez-Salazar, Rami Doueiri, Antonio Valentin, Margherita Rosati, Xintao Hu, Brandon F. Keele, et al. “Control of Heterologous Simian Immunodeficiency Virus SIVsmE660 Infection by DNA and Protein Coimmunization Regimens Combined with Different Toll-Like-Receptor-4-Based Adjuvants in Macaques.” J Virol 92, no. 15 (August 1, 2018). https://doi.org/10.1128/JVI.00281-18.

Full Text

Barouch, Dan H., Frank L. Tomaka, Frank Wegmann, Daniel J. Stieh, Galit Alter, Merlin L. Robb, Nelson L. Michael, et al. “Evaluation of a mosaic HIV-1 vaccine in a multicentre, randomised, double-blind, placebo-controlled, phase 1/2a clinical trial (APPROACH) and in rhesus monkeys (NHP 13-19).” Lancet 392, no. 10143 (July 21, 2018): 232–43. https://doi.org/10.1016/S0140-6736(18)31364-3.

Full Text

Scinto, Hanna B., Sandeep Gupta, Swati Thorat, Muhammad M. Mukhtar, Anthony Griffiths, Jennifer Delgado, Elizabeth Plake, et al. “Novel Strategy To Adapt Simian-Human Immunodeficiency Virus E1 Carrying env from an RV144 Volunteer to Rhesus Macaques: Coreceptor Switch and Final Recovery of a Pathogenic Virus with Exclusive R5 Tropism.” J Virol 92, no. 14 (July 15, 2018). https://doi.org/10.1128/JVI.02222-17.

Full Text

Bekker, Linda-Gail, Zoe Moodie, Nicole Grunenberg, Fatima Laher, Georgia D. Tomaras, Kristen W. Cohen, Mary Allen, et al. “Subtype C ALVAC-HIV and bivalent subtype C gp120/MF59 HIV-1 vaccine in low-risk, HIV-uninfected, South African adults: a phase 1/2 trial.” Lancet Hiv 5, no. 7 (July 2018): e366–78. https://doi.org/10.1016/S2352-3018(18)30071-7.

Full Text

Manoharan, Vinoth K., Sunil K. Khattar, Celia C. LaBranche, David C. Montefiori, and Siba K. Samal. “Modified Newcastle Disease virus as an improved vaccine vector against Simian Immunodeficiency virus.” Sci Rep 8, no. 1 (June 12, 2018): 8952. https://doi.org/10.1038/s41598-018-27433-x.

Full Text

Pardi, Norbert, Michael J. Hogan, Martin S. Naradikian, Kaela Parkhouse, Derek W. Cain, Letitia Jones, M Anthony Moody, et al. “Nucleoside-modified mRNA vaccines induce potent T follicular helper and germinal center B cell responses.” J Exp Med 215, no. 6 (June 4, 2018): 1571–88. https://doi.org/10.1084/jem.20171450.

Full Text

Vaccari, Monica, Slim Fourati, Shari N. Gordon, Dallas R. Brown, Massimilano Bissa, Luca Schifanella, Isabela Silva de Castro, et al. “HIV vaccine candidate activation of hypoxia and the inflammasome in CD14+ monocytes is associated with a decreased risk of SIVmac251 acquisition.” Nat Med 24, no. 6 (June 2018): 847–56. https://doi.org/10.1038/s41591-018-0025-7.

Full Text

Cai, Hui, Roushu Zhang, Jared Orwenyo, John Giddens, Qiang Yang, Celia C. LaBranche, David C. Montefiori, and Lai-Xi Wang. “Multivalent Antigen Presentation Enhances the Immunogenicity of a Synthetic Three-Component HIV-1 V3 Glycopeptide Vaccine.” Acs Cent Sci 4, no. 5 (May 23, 2018): 582–89. https://doi.org/10.1021/acscentsci.8b00060.

Full Text

Song, Hongshuo, Elena E. Giorgi, Vitaly V. Ganusov, Fangping Cai, Gayathri Athreya, Hyejin Yoon, Oana Carja, et al. “Tracking HIV-1 recombination to resolve its contribution to HIV-1 evolution in natural infection.” Nat Commun 9, no. 1 (May 15, 2018): 1928. https://doi.org/10.1038/s41467-018-04217-5.

Full Text

Pages